12/27/2022 0 Comments Fiji imagej mp4![]() ![]() ![]() Tomancak (2009) "Globally optimal stitching of tiled 3D Global optimization scheme to minimize propagatingįusing options (blending) new tile coordinates also Pair-wise registration to sub-pixel resolution (FFT) Reconstruct big image stacksarbitrary spacing Single image from sectioning experiment MIN Z-projection thru 300 sections Original Image 3D Median (3 x 3 x 3 pixel kernel)Įffective for feature enhancement, removing some artifacts Works on stacks as well as individual imagesģD kernel operations can be readily performed Helpful to have numeric ordering of image stack Open source enables insight into algorithm construction Wide proliferation enables sharing/verification of workflow Macro language enables rapid construction of automation Extensive suite of scripts/plug-ins developed by ![]() Open source (GNU) built off of ImageJ2 (BSD)Ĭlose collaboration with other SciJava/open microscopyĬore code is robust comparatively fast executionįIJI has very effective filter suite for data post-processing, FIJI has lots of plug-ins to assist with 3D data analysis Emphasis on filters and techniques useful forįIJI (and ImageJ) = Image processing package ![]()
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